Immunochemical and kinetic evidence that heparin enhances aprotinin activity.
نویسنده
چکیده
Both immunochemical and kinetic evidence suggest that the increase in aprotinin activity in the presence of heparin is not due to an increase in the active form of aprotinin, but rather to a qualitative change in the aprotinin molecule which would lead to an increase in aprotinin activity.
منابع مشابه
Effects of dextran sulfate on aprotinin activity.
Dextran sulfate has an action on aprotinin activity similar to that seen with heparin: preincubation of dextran sulfate with aprotinin enhanced the inhibitory effect of aprotinin on the esterolytic activity of trypsin, but did not change the effect on the proteolytic activity of trypsin or on the esterolytic and proteolytic activities of chymotrypsin. The enhancing effects of heparin and dextra...
متن کاملThe enhancing effect of gelatin on aprotinin activity.
Following preincubation of aprotinin with gelatin, the inhibitory effect on the trypsin activity was enhanced under the same condition as in the case of enhanced antigen-antibody reaction of aprotinin in gel in the presence of gelatin. In the immunochemical titration of aprotinin, gelatin treatment decreased the value of the equivalence point of aprotinin. Immunoprecipitation of aprotinin prein...
متن کاملIn vivo and in vitro evaluation of the heparin management test versus the activated coagulation time for monitoring anticoagulation level in aprotinin-treated patients during cardiac surgery.
INTRODUCTION Monitoring whole blood anticoagulation therapy with the activated coagulation time (kaolin ACT) and the heparin management test (HMT) were correlated in vivo with the plasma anti-activated factor X (anti-Xa) heparin concentration in patients who received variable doses of aprotinin and in vitro in the presence of increasing concentrations of aprotinin. METHODS In 38 elective card...
متن کاملReheparinisation requirements after cardiopulmonary bypass in patients treated with aprotinin.
OBJECTIVE To determine reheparinisation requirements following protamine neutralisation after the discontinuation of cardiopulmonary bypass in a group of patients receiving "low dose" aprotinin compared with a control group. DESIGN Randomised, placebo controlled study. SETTING Regional cardiothoracic unit within a district general hospital. PATIENTS 20 patients were consecutively allocate...
متن کاملComparison of Celite and Kaolin Based Heparin and Protamine Dosing Assays During Cardiac Surgery: The In Vitro Effect of Aprotinin
Both aprotinin and in vitro heparin and protamine dosing assays (RxDx System) have been shown to reduce postoperative bleeding in the cardiac surgery patient. While celite activated clotting time tests in the presence of heparin are artificially prolonged by aprotinin, the kaolin based ACT is not. In order to optimize heparin anticoagulation management during cardiac surgery when aprotinin is u...
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عنوان ژورنال:
- Japanese journal of pharmacology
دوره 38 4 شماره
صفحات -
تاریخ انتشار 1985